::SecurityProtocol = ::SecurityProtocol -bor 3072 # installed (.NET 4.5 is an in-place upgrade). # We use this variable for future REST calls. # If required, add the repository access credential here $NugetRepositoryUrl = "INTERNAL REPO URL" # Should be similar to what you see when you browse Your internal repository url (the main one).
Alternative to serial cloner download#
# This url should result in an immediate download when you navigate to it # $RequestArguments.Credential = $NugetRepositor圜redential # ("password" | ConvertTo-SecureString -AsPlainText -Force) # If using CCM to manage Chocolatey, add the following: $ChocolateyDownloadUrl = "$($NugetRepositoryUrl.TrimEnd('/'))/package/chocolatey.1.1.0.nupkg" #ALTERNATIVE TO SERIAL CLONER DOWNLOAD#
Alternative to serial cloner archive#
Zip to the filename to handle archive cmdlet limitations # Ensure Chocolatey is installed from your internal repository # $Chocolate圜entralManagementServiceSalt = "servicesalt" # $Chocolate圜entralManagementClientSalt = "clientsalt" # $Chocolate圜entralManagementUrl = " # ii. If (-not (Get-Command choco.The CloneMiner cDNA Library Construction Kit is designed to construct high-quality cDNA libraries without the use of traditional restriction enzyme cloning methods. This novel technology combines the performance of SuperScript II Reverse Transcriptase with the Gateway Technology. Single-stranded mRNA is converted into double stranded cDNA containing attB sequences on each end. Through site-specific recombination, attB-flanked cDNA is cloned directly into an attP-containing donor vector without the use of restriction digestion or ligation. The resulting Gateway entry cDNA library can be screened with a probe to identify a specific entry clone. Alternatively, the entire entry cDNA library can be shuttled into a Gateway destination vector to generate an expression library.įeatures of the CloneMiner cDNA Library Construction Kit include: This clone can be transferred into the Gateway destination vector of choice for gene expression and functional analysis.SuperScript II reverse transcriptase for efficient conversion of mRNA into cDNA.Biotin-attB2-Oligo(dT) Primer for poly(A) mRNA binding and incorporation of the attB2 sequence to the 3' end of cDNA.attB1 Adapter for ligation of the attB1 sequence to the 5' end of double-stranded cDNA.attP-containing vector (pDONR 222) for recombination with attB-flanked cDNA to produce an entry library through the Gateway BP recombination reactionĪdvantages of the CloneMiner cDNA Library Construction Kit.Using CloneMiner cDNA Library Construction Kit offers the following advantages:.Produces high yields of quality, double-stranded cDNA.Eliminates use of restriction enzyme digestion and ligation allowing cloning of undigested cDNA.#ALTERNATIVE TO SERIAL CLONER DOWNLOAD#.#ALTERNATIVE TO SERIAL CLONER SOFTWARE#.
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